| abstract |
The present invention relates to a kit for identifying the status of pulmonary nodules and/or lung cancer and its application, the kit includes a kit for detecting the methylation level of a biomarker gene or a corresponding fragment in a biological sample from a subject A pair of primers; the pair of primers is used to carry out a PCR amplification reaction using a bisulfite-treated biomarker gene or a fragment thereof as a template; the biomarker gene is selected from the group consisting of APC, DAPK, FHIT, FOXL2, HOXA9, MGMT, P16, One or more of PTGER4, RASSF1A, SHOX2, SOX17 and ZAR1. The present invention improves the sensitivity and specificity of lung nodules and lung cancer detection by jointly detecting the methylation levels of biomarker genes or corresponding fragments, thus ensuring the correctness and reliability of the detection results The prediction, diagnosis and evaluation of lung cancer and lung cancer provide a fast, reliable and accurate new way. |