http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109022472-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1a7e89b0bed9079e309e1f886ea9e25d |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-22 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y114-13013 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0073 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2018-08-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c3189f6f1ecb82936446676dc9586d89 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_205a18dd4381871b6e7f39bf0e3731a4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a60702c7a7df0bbb87e4f94c8699a3e6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e8681dedb590d0ffa8cc477339d081cc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b89b7322e408d2db651d0458d89ed147 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e75655a241a073b05a5099e90a5daaea |
| publicationDate | 2018-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-109022472-A |
| titleOfInvention | The construction of recombinant vector of -1 α '-hydroxylase gene of 25(OH)VD containing reinder and expression, isolation and purification method |
| abstract | It the present invention relates to the recombinant vector of -1 α '-hydroxylase gene of 25(OH)VD containing reinder and its encodes the expression of albumen, isolate and purify the method with determination of activity: 1) according to -1 α '-hydroxylase gene of 25(OH)VD and e. coli codon Preference annotated out from the genome of reinder, -1 α '-hydroxylase gene sequence of reinder 25(OH)VD after being optimized, -1 α '-hydroxylase gene sequence of 25(OH)VD for similarly obtaining the roe deer after optimizing according to e. coli codon Preference and sheep, to as control；2) primer of design is utilized, pass through gibson connection method, reinder, roe deer, -1 α '-hydroxylase gene of sheep 25(OH)VD, cortex ferredoxin gene and NADPH- cortex ferredoxin reductase gene after optimization is implemented in coli expression carrier pET-28a respectively, obtains recombinant vector.Final detection, the catalytic activity of the bacterial strain expression acquisition enzyme of -1 α '-hydroxylase gene of 25(OH)VD containing reinder are 2 times of sheep, are 1.5 times of roe deer. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021147857-A1 |
| priorityDate | 2018-08-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 57.