http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108998505-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
| filingDate | 2018-07-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108998505-B |
| titleOfInvention | Gene polymorphism site detection technology and kit thereof |
| abstract | The invention provides a method for detecting gene polymorphism sites by using a mismatch probe. In a recombinase polymerase amplification reaction system, one or two specific mismatch probes are added, and according to the influence of the mismatch between the probes and a template on the enzyme digestion efficiency, the characteristic can distinguish gene polymorphism sites through fluorescent signals on the probes, so that the method has important application value in the fields of disease detection and the like. The mispairing probe takes a genotyping site as a No. 1 site, at least one base which is mispaired with a sequence to be detected is artificially introduced into a No. 2-4 site at the upstream (5' direction) of the probe, and the mispaired site can increase the specificity of the probe in detecting the single nucleotide polymorphism of the gene. The invention also provides a specific probe and a primer for detecting the single nucleotide polymorphism and a detection kit component based on recombinase polymerase amplification. |
| priorityDate | 2018-07-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 384.