abstract |
One plant of aristolochic acid A monoclonal antibody hybridoma cell strain and its application, belong to food safety field of immunodetection.The present invention includes one plant of aristolochic acid A monoclonal antibody hybridoma cell strain SS0709, is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and deposit number is CGMCC No.14688, and the deposit date is on September 5th, 2017.Aristolochic acid A monoclonal antibody, is CGMCC No.14688 by the deposit number, and aristolochic acid A monoclonal antibody hybridoma cell strain SS0709 secretion generates.The application of the aristolochic acid A monoclonal antibody, for aristolochic acid A analysis detection in Chinese medicine.The monoclonal antibody of cell strain secretion of the present invention has preferable specificity and detection sensitivity (IC to aristolochic acid A 50 Value is 4ng/mL), it can be achieved that being detected to aristolochic acid A in Chinese medicine, the immune detection for aristolochic acid A in Chinese medicine provides raw material, has practical application value. |