http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108982457-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_47c37935f119cad24aeba657d69c4408 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d496576561159f683d5fd7c887538114 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6439 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6854 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate | 2018-08-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1f2de437573a6bbb8f393bb1a3cc56da http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aee822294ad2af7590b764cf4241434f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b3933a5e0ce3002b13e3357223056be9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_365b7801e7f5da1e58dd94d0e7882e39 |
| publicationDate | 2018-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108982457-A |
| titleOfInvention | Method for detecting antisperm antibodies in single spermatozoa using fluorescent dyes |
| abstract | The present invention relates to a kind of method that uses fluorescent dye to detect the anti-sperm antibody of single sperm, it is characterized in that: comprise the following steps: (1) adopt isothiocyanate as fluorescein to mark immune anti-human IgG and IgA antibody, as secondary antibody ; (2) Separation of sperm and seminal plasma; (3) Incubate the spermatozoa separated in step (2) with the immune anti-human IgG and IgA of step (1) at 37°C; (4) add propidium iodide staining subsequently The sperm is stained, the propidium iodide can be specifically stained with the dead sperm nucleus, and the living sperm is not stained; (5) the sperm is detected by flow cytometry. Compared with the prior art, the present invention has the following advantages: The invention provides a method for detecting antisperm antibodies by flow cytometry, so that the present invention is a simple, rapid, sensitive and accurate detection method, suitable for detecting antisperm antibodies bound to the surface of sperm, and detecting and distinguishing dead and living sperm. |
| priorityDate | 2018-08-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 59.