| abstract |
The invention belongs to the technical field of biological detection, and in particular relates to a kit for detecting H7N9 avian influenza virus and a method thereof. The kit includes a first forward primer, a first reverse primer, and a first probe for amplifying the H7 gene, and a second forward primer, a second reverse primer, and a second probe for amplifying the N9 gene. Two probes; wherein, the sequence of the first forward primer is shown in SEQ ID NO.1, the sequence of the first reverse primer is shown in SEQ ID NO.2, and the sequence of the first probe As shown in SEQ ID NO.3, the sequence of the second forward primer is shown in SEQ ID NO.4, the sequence of the second reverse primer is shown in SEQ ID NO.5, the second probe The sequence of the needle is shown in SEQ ID NO.6. The kit can be applied to clinical instant and rapid detection of H7N9 avian influenza virus. |