http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108977506-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6834 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6841 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6834 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6841 |
| filingDate | 2018-08-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108977506-B |
| titleOfInvention | Method for rapidly screening microbial strains generating gonyautoxin and digoxin labeled DNA probe used in method |
| abstract | The invention relates to the technical field of marine organisms, in particular to a method for rapidly screening a microorganism strain generating gonyautoxin and a digoxin labeled DNA probe used by the method, wherein the probe used by the method is single-stranded DNA with the length of 248 bases complementary with the bases of DNA to be detected, and the 3' end of the probe is labeled by digoxin. Through colony in-situ spot molecular hybridization, the probe can specifically detect whether the DNA sample of the strain to be detected has gonyautoxin synthesis (sxTS) gene nucleic acid, and screen out the strains which are positive for virus production. The method utilizes the in-situ extract of the strain DNA to carry out colony spot hybridization, and can complete 96 samples for screening within 8-10 hours. The method is rapid, strong in specificity and high in accuracy. |
| priorityDate | 2018-08-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 104.