http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108956791-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6848 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 |
| filingDate | 2017-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108956791-B |
| titleOfInvention | Method for screening protein biomarkers in large scale |
| abstract | The invention discloses a method for screening protein biomarkers in a large scale, which comprises the steps of (1) homogenizing and cracking a biological tissue control sample and a reference sample (pathological sample); (2) carrying out operations such as enzymolysis and desalting on the lysate; (3) carrying out protein identification by using a LC-MS/MS liquid chromatography-mass spectrometry method; (4) analyzing the abundance change of the sample protein by comparing the mass spectrometry times or mass spectrometry peak intensity, calculating the integral of the signal intensity of each peptide segment on LC-MS based on MS1, and correcting the data in large scale based on the identification result of MS 2; (5) and screening protein biomarkers which are obviously up-regulated or down-regulated according to the corrected data analysis result. The method does not need expensive marking reagent, has low cost, large screening quantity at one time and accurate screening result. |
| priorityDate | 2017-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 164.