http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108956566-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6432 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-682 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54326 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-682 |
| filingDate | 2018-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-10-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-10-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108956566-B |
| titleOfInvention | Kit and method for detecting polychlorinated biphenyl by up-conversion fluorescence based on nicking endonuclease and hybrid chain reaction dual amplification |
| abstract | The invention belongs to the technical field of immunoassay for environmental safety detection, and relates to an upconversion fluorescence detection polychlorinated biphenyl kit based on nicking endonuclease and hybridization chain reaction dual amplification and a detection method. The kit comprises the following components: (1) magnetic microparticles; (2) an aptamer that specifically recognizes polychlorinated biphenyl PCB 72/106; (3) a complementary DNA at least partially complementary to the aptamer; (4) up-converting the nanoparticles; (5) a pair of hairpin DNAs for use in a hybrid chain reaction; (6) a nicking endonuclease; wherein the complementary DNA is capable of priming the pair of hairpin DNAs for a hybrid chain reaction. The method of the invention can detect the polychlorinated biphenyl PCB72/106 with high sensitivity. |
| priorityDate | 2018-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 29.