| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_33a62710796e131599043055d399597f |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-80 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-39 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-78
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-84
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-645
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-72 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 |
| filingDate |
2017-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9b5875b1f1136f8949fa65d886597249
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_af54d0f091f89aba39b5cfa64c2c644a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3c48cdeae59ef18d70d6db9a9716ec15
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0ad689dad892cb6539fa51c0502c13ee |
| publicationDate |
2018-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-108949869-A |
| titleOfInvention |
Carbon source-free suppression Pichia pastoris expression system, its establishment method and application |
| abstract |
The invention relates to a high-expression, carbon-source-free, enhanced methanolotrophic yeast expression system, its establishment method and application. The present invention discloses a method for eliminating the single methanol carbon source dependence and carbon source severe repression of methanol-inducible promoters. By artificially modifying the transcriptional regulation genetic circuit in methanolotrophic yeast cells, the transcriptional intensity of the promoter is improved and its regulation is changed. model. The yeast expression system and expression method of the present invention make the promoters that are originally dependent on methanol induction and have carbon source repression no longer depend on methanol, and can also efficiently express foreign polypeptides under other carbon source conditions, and the highest expression level can reach wild type AOX1 promoter 5-fold under methanol conditions. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109679939-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114891824-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114891824-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111349576-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108728477-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109929769-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109929769-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111349576-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115322998-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110079546-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110079546-B |
| priorityDate |
2017-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |