http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108949805-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8213 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
filingDate | 2017-05-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-07-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-07-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-108949805-B |
titleOfInvention | Plant genome multi-site editing vector pCXUN-CAS9-RGR |
abstract | The invention belongs to the technical field of plant genetic engineering, and particularly relates to a plant genome multi-site editing vector pCXUN-CAS 9-RGR. The invention utilizes CRISPR/CAS9 technology to combine with RGR to efficiently transform rice knockout related genes to obtain a vector, and the vector is named as pCXUN-CAS 9-RGR. The carrier comprises a carrier shown as a sequence table SEQ ID NO: 6. The invention utilizes RGR method to mutate rice target gene to obtain high-efficiency transformation carrier, which is a unitary carrier, the editing carrier of the invention can be used for directional multi-target site mutation rice target gene, researching the function and action of related gene in rice, and constructing corresponding gene mutant and large fragment deletion. The vector of the invention has high transformation efficiency and targeting mutation efficiency, and can be stably inherited and applied in rice. |
priorityDate | 2017-05-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.