http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108949794-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K48-0025 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P35-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P35-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K48-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2018-07-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108949794-B |
| titleOfInvention | TALE expression vector and rapid construction method and application thereof |
| abstract | The invention discloses a TALE expression vector and a rapid construction method and application thereof, wherein the TALE vector is a final functional TALE expression vector formed by assembling an assembled annular 5-linked body and a TALE framework vector, and the assembled annular 5-linked body is formed by carrying out enzyme digestion-connection reaction on 62 monomers. The TALE expression vector can be rapidly assembled into a customized TALE vector in one day, eliminates the obstacles of TALE application in the prior art, and can activate the expression of an exogenous reporter gene and an endogenous gene. The invention discloses a method for rapidly constructing TALE vectors by TALE expression, which is characterized in that 62 new monomers are prepared and can be used for rapidly assembling customized TALE vectors in one day, the feasibility of the method is verified by designing and assembling 9 TALE vectors aiming at transcription factor gene HNF4 alpha and E47 promoters, and two TALE vectors which can be used for liver cancer and pancreatic cancer differentiation therapy are identified. |
| priorityDate | 2018-07-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 154.