| abstract |
The present invention relates to a kind of stem cell medias, including basal medium and additive, additive includes 1~200 μ g/mL of recombinant transferrin, 1~300 μ g/mL of recombinant albumin, 0.1~200ng/mL of recombinant cytokine, free fatty acid 10~300 μ g/mL, 0.1~10mg/mL of protease inhibitors, 20~300 μ g/mL of vitamin, 0.5~10mg/mL of zinc sulfate, 0.01~1mmol/L of Tretinoin, 10~60nmol/L of sodium selenite, 5~100 μ g/mL of niacinamide；The present invention discloses a kind of stem cell culture methods, i.e., carry out dimensional culture to stem cell using serum-free stem cell media provided by the present invention.Culture medium and cultural method provided by the invention expand the applicability of culture medium, while providing stable growing environment for stem cell, significantly improve stem cell culture efficiency, reduce the use cost of culture medium. |