http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108931513-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2800-10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6854 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate | 2018-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108931513-B |
| titleOfInvention | Method and kit for in vitro detection of MUSK-Ab and LRP4-Ab |
| abstract | The invention relates to a method and a kit for in vitro detection of MUSK-Ab and LRP 4-Ab. The method comprises the following steps: taking 293T cells, and transfecting the 293T cells with a pCMV6-AC-GFP-MuSK recombinant plasmid and/or a pCMV6-AC-GFP-LRP4 recombinant plasmid; adding paraformaldehyde into transfected cells transfected with the pCMV6-AC-GFP-MuSK recombinant plasmid and/or transfected cells transfected with the pCMV6-AC-GFP-LRP4 recombinant plasmid, and fixing for 15-30 min; after washing with phosphate buffer solution, adding bovine serum albumin and TritonX-100 for sealing and permeating for 2-3 h; adding the serum to be detected into the transfection cells of the closed and permeable pCMV6-AC-GFP-MuSK recombinant plasmid and/or the transfection cells of the closed and permeable pCMV6-AC-GFP-LRP4 recombinant plasmid, incubating for 45min to 2h at 24 to 28 ℃, washing by phosphate buffer solution, and adding Alexa Fluor TM 568 labeled goat anti-human IgG is incubated at 24-28 ℃ for 45 min-2 h, washed by phosphate buffer solution, excited by blue excitation light and green excitation light of a fluorescence microscope, observed, photographed and recorded. The used reagents are all conventional reagents, and the detection cost is low without depending on expensive kits. |
| priorityDate | 2018-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 46.