| abstract |
The invention discloses double colour developing qualitative, quantitative immuno-chromatographic test paper strips of fluorescent microsphere-colloidal gold of detection antibiotic and preparation method thereof, in turn overlap joint pastes filter paper, sample pad, is coated with fluorescent microsphere-antibiotic monoclonal antibody complex and colloidal gold-antibiotic monoclonal antibody complex fiberglass packing, nitrocellulose filter and blotting paper on bottom plate, and antibiotic coupled antigen is coated on the nitrocellulose filter as detection line and is coated with anti-mouse antibody as nature controlling line.Detection line colour developing is more shallow than nature controlling line colour developing, then it is the positive that antibiotic, which qualitatively judges, in sample, and positive test strips are inserted directly into fluorescence and read in instrument, reads instrument and the fluorescence signal of fluorescent microsphere color development system quantizes, realization quantitative detection.Present invention is mainly used for the qualitative and quantitative detections of antibiotic in food safety detection. |