http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108912217-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-43509 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-435 |
filingDate | 2018-07-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-108912217-B |
titleOfInvention | Pre-fibrin gene Cq-Pfn1 for promoting WSSV infection and preparation method and application thereof |
abstract | A pre-fibrin gene Cq-Pfn1 for promoting WSSV infection and a preparation method and application thereof relate to gene cloning and gene engineering expression. Connecting the Cq-Pfn1 gene to a pMD18-T vector, analyzing to obtain an ORF reading frame to obtain a Cq-Pfn1ORF gene sequence, and performing sequence comparison analysis, wherein the Cq-Pfn1 has high conservation compared with other species; constructing a gene engineering recombinant expression vector of the procambarus clarkia pre-fibrin pGEX-4T-1-Cq-Pfn 1; introducing the obtained recombinant prokaryotic expression vector pGEX-4T-1-Cq-Pfn1 into a host cell for induction expression to obtain an expression product, and obtaining a high-purity recombinant protein GST-Cq-Pfn1 through affinity chromatography. The procambarus clarkia pre-fibrin Cq-Pfn1 is applied to the targeted prevention and treatment of white spot syndrome diseases and the medicine for preventing and treating white spot syndrome. |
priorityDate | 2018-07-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 169.