http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108893493-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2740-15043 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-867 |
| filingDate | 2018-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108893493-B |
| titleOfInvention | Method for improving transfection efficiency of NK cells |
| abstract | The invention relates to the technical field of NK cell in-vitro amplification, and particularly relates to a method for improving NK cell transfection efficiency. According to the method for improving the transfection efficiency of the NK cells, when the NK cells are infected by lentiviruses, the lentivirus vector containing CAR is added in the first infection, and the infection assisting agent 1 is added at the same time, and the lentivirus vector containing CAR is added in the second infection, and the infection assisting agent 2, the infection assisting agent 3 and the infection assisting agent 4 are added at the same time; the infection assistant 1 is a polybrene-containing solution; the infection adjuvant 2 is a solution containing IL 2; the infection adjuvant 3 is a solution containing IL 12; the infection adjuvant 4 is a solution containing PHA. According to the Car-NK transfection method provided by the invention, different infection-assisting agents are added in the transfection process, so that the transfection efficiency of NK cells is improved, and the lentivirus transfection efficiency of the NK cells can be improved from less than 10% to 50% -60%. |
| priorityDate | 2018-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 60.