http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108872166-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6441 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6458 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-643 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2018-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108872166-B |
| titleOfInvention | Method for realizing three-dimensional visualization of rape seed single cells and oil drops by double fluorescence labeling |
| abstract | The invention discloses a method for realizing three-dimensional visualization of rape seed single cells and oil drops by double fluorescence labeling, which comprises the following steps: (1) dissecting and slicing rape seeds; (2) carrying out Nile red staining and DiO staining on the rape seed sections; (3) preparing a rape seed double-fluorescence labeling section specimen; (4) double-fluorescence deep scanning by a laser confocal microscope; (5) and (3) three-dimensional reconstruction of the rape seed cell double-fluorescence labeling signal. The invention utilizes double fluorescence labeling, improves and further optimizes the specific type and specific dyeing process of the dye, the scanning conditions adopted by the subsequent laser confocal deep scanning, and the like, can effectively solve the problems of difficult three-dimensional visualization processing and the like of the rape seed cells or the oil drops in the rape seed cells compared with the prior art, and can realize the accurate measurement of the oil drops and the cell volume. |
| priorityDate | 2018-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 20.