http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108866224-B

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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6895
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6895
filingDate 2018-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2022-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2022-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-108866224-B
titleOfInvention Multiplex PCR (polymerase chain reaction) kit for detecting thirteen transgenic soybeans and detection method
abstract The invention discloses a multiplex PCR kit and a detection method for detecting thirteen transgenic soybeans, which can quickly detect 13 transgenic soybeans approved to be imported by the Ministry of agriculture of China, including MON87701, GTS40-3-2, MON89788, CV127, A2704, A5547, DP356043, DP305423, MON87769, MON87708, 305423 multiplied by 40-3-2, MON87701 multiplied by MON89788 and MON 87705; the multiple PCR kit disclosed by the invention is based on a public primer mediated multiple PCR method, and comprises the detection of a public primer, multiple primers and a multiple PCR system; the newly designed annealing temperature method improves the specificity and the sensitivity of a multiplex PCR system, has compatibility, can continuously increase detection targets, and is favorable for improving the detection flux of the multiplex PCR. The method has the advantages of high sensitivity, economy, rapidness, simple operation and the like, and can be applied to food detection.
priorityDate 2018-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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