http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108866141-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0908d6a1057eac8a59f0fde33f1672e3 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P33-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P33-00 |
| filingDate | 2018-08-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_39f76c99019682e341881173f2e4e3d5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_85095ff4943b66311a4f2fe7f55a13c2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9a9e4724b59972fc13a49768274f453c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bd6b76fc2f7bae467493442feb5221f1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b99cc67db3f42e553a1c339405385dce http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7762fe71d842830ce2eed6e5cc7ce4e0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b8ee12ad788cc440f3afd9e44cc83399 |
| publicationDate | 2018-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108866141-A |
| titleOfInvention | A kind of enzymatic synthesis method of ursodesoxycholic acid |
| abstract | Disclosed by the invention is a kind of enzymatic synthesis method of ursodesoxycholic acid, when solving in the prior art using chenodeoxycholic acid preparation ursodesoxycholic acid, usually occurs the case where 3 hydroxyls of simultaneous oxidation in oxidation-reduction process, the problem for causing yield and purity not high.The present invention includes step 1, and the 7-KLCA as shown in formula one is made；Step 2,7-KLCA is added in phosphate buffer, reaction solution is made, L MALIC ACID sodium, malic dehydrogenase, 7- β steroid dehydrogenase are added in reaction solution, control reaction temperature 25~30 DEG C, reaction pH be 7.0~7.5 under the conditions of, reaction until 7-KLCA residual be lower than 0.1%, after the reaction was completed, add alkali to be warming up to 60~80 DEG C to stir 1~3 hour, then it is cooled to room temperature, is filtered, filtrate acid adding crystallizes to obtain ursodesoxycholic acid.The advantages such as the present invention has yield and purity is high, and production cost is low. |
| priorityDate | 2018-08-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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