| abstract |
The invention provides a method for extracting water-soluble dietary fiber from highland barley bran, comprising the following steps: 1) mixing highland barley bran with water, adjusting the pH to 5.5-8.0 to obtain mixture I; Add peak α-amylase to mixture I, and carry out enzymolysis at a temperature of 90-100° C.; 3) centrifuge the enzymolyzate obtained in step 2), mix and stir the filter residue with water, and adjust the pH to 7.0-9.5 to obtain Mixture II; 4) adding alkaline protease to the mixture II obtained in step 3), and performing enzymolysis at a temperature of 50-60°C; 5) centrifuging the enzymolyzate obtained in step 4), mixing the filter residue with water, Adjust the pH to 4.5-5.0 to obtain mixture III; 6) add cellulase to the mixture III obtained in step 5), and perform enzymolysis at a temperature of 40-50°C; 7) perform enzymolysis on the enzymolyzate obtained in step 6) Separation, the filtrate is water-soluble dietary fiber extract. The method provided by the invention can efficiently extract the water-soluble dietary fiber in the highland barley bran. |