http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108866096-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8218 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8205 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H6-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-29 |
| filingDate | 2018-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-11-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-11-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108866096-B |
| titleOfInvention | Method for establishing virus-induced gene silencing system on rosa multiflora plant |
| abstract | The invention discloses a method for establishing a virus-induced gene silencing system on a rosa plants in a field, which relates to the field of genetic engineering for improving the shape of rosa plants, and comprises five steps of determining an inoculation period, preparing an infection liquid, inoculating a virus vector, treating after inoculation and testing the gene silencing efficiency, wherein the concentration of the agrobacterium carrying the virus vector is increased to OD in the preparation of the infection liquid 600 1.5-2.0, which ensures that the agrobacterium carrying the virus vector in the inoculation area has enough survival base number under natural conditions, and improves the infection efficiency of the whole experiment; the low-concentration Silwet-77 component is added in the preparation of the infection liquid, so that the hydrophilicity of the surface of the plant material is increased, the infection efficiency of the infection liquid is improved, meanwhile, the low-concentration component does not produce toxic action on the plant material, the pollution rate is low, and the environment is protected; the method not only saves the cost, but also improves the gene silencing efficiency, and the gene silencing efficiency of the processed sample reaches more than 87.5 percent. |
| priorityDate | 2018-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 36.