http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108866013-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2740-15051 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2531-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M41-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M29-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M41-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M23-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M23-14 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M3-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
| filingDate | 2018-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108866013-B |
| titleOfInvention | Method for large-scale production of lentivirus |
| abstract | The invention discloses a method for producing lentivirus in large scale, which comprises the following steps: step one, cell culture; step two, culturing the cells in a culture bag; step three, amplifying the lentiviruses on a large scale; step four, purification; wherein, the cell culture bags in the second step and the third step are all placed in a shaking table and cultured in a constant temperature box; the culture conditions of the cell culture bag are that the temperature is 37 ℃, the dissolved oxygen is set to be 60 percent, the PH value is 7.1, the swing speed is 10-20rpm, and the angle of a shaking table is 7-9 degrees. The invention improves the cell growth environment, reduces the damage to cells during aeration, increases the dissolved oxygen effect, reduces the damage of mechanical shearing force to the cells, and improves the growth density of the cells, thereby improving the total yield of the cells and further improving the yield of lentiviruses. |
| priorityDate | 2018-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 26.