http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108865997-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-999 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-115 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-119 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0622 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-079 |
filingDate | 2018-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-02-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-02-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-108865997-B |
titleOfInvention | Culture medium and culture method for in vitro culture of astrocytes |
abstract | The invention discloses a culture medium and a culture method for in vitro culture of astrocytes, and belongs to the field of cell culture. According to the invention, the epidermal growth factor, the fibroblast growth factor, SAG and Purmorphamine are added into the astrocyte culture medium, so that the in-vitro culture time of astrocytes can be obviously prolonged, the passage times are increased, the purity is improved, and the original characteristics of astrocytes can be maintained. The inventors found that the medium of the present invention can be used at alternate generations and maintained the astrocytes for passage to 13. Culturing until the purity of the astrocytes of 12 generations is more than 95%, and keeping the original characteristics of the astrocytes. |
priorityDate | 2017-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 117.