http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108865974-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-115 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2760-16051 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-11 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0602 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-00 |
| filingDate | 2018-06-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108865974-B |
| titleOfInvention | Tilapia mossambica brain cell line and its application |
| abstract | The invention discloses a kind of construction method of Tilapia mossambica brain cell line, it the steps include: that fresh and alive Tilapia mossambica disinfection is taken to take brain tissue, shred block, after collagen enzymatic treatment, trypsase is added；It is sufficiently suspended with containing culture solution, carries out originally culture；It when cell covers with bottom of bottle, is digested with pancreas enzyme -EDTA, is inoculated with secondary culture；After 60 generation of secondary culture, is handled in logarithmic growth phase addition colchicine and carry out karyotyping.The preparation method of Tilapia mossambica brain cell line disclosed by the invention, method are simple and convenient；And the Tilapia mossambica brain cell line prepared with this method, can carry out prolonged secondary culture, very sensitive to Tilapia mossambica hemorrhage virus, can be widely applied in experiment. |
| priorityDate | 2018-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 74.