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filingDate 2018-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2019-08-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2019-08-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-108865119-B
titleOfInvention Light-up fluorescent compound for labeling cancer cells under freezing conditions, preparation method and application thereof
abstract The invention relates to the technical field of biosensors, in particular to a light-up fluorescent compound used for labeling cancer cells under freezing conditions, a preparation method and application thereof. The preparation method of the fluorescent compound includes the following steps: compound A is dissolved in a dimethyl alcohol amine compound, a basic compound is added, the reaction is carried out at 50-90° C. for 6-24 hours, and the precipitate is collected. The fluorescent compound has a selective targeting effect on cancer cells, and can effectively distinguish cancer cells from normal cells when the cancer cells and normal cells are cultured under the same conditions or mixed culture; and under the freezing condition of ‑40°C, the fluorescent compounds can instantly It is quick and convenient to "light up" cancer cells to distinguish them from normal cells, and can be used for visual monitoring of cancer cells under freezing conditions according to actual needs.
priorityDate 2018-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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