http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108849523-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-008 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
| filingDate | 2018-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2019-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108849523-B |
| titleOfInvention | A kind of Calotropis gigantea tissue culture and rapid propagation method |
| abstract | The present invention provides a kind of Calotropis gigantea tissue culture and rapid propagation methods, belong to plant tissue culture technical field.The method of the invention is inoculated in primary inducing culture after disinfection the following steps are included: 1) using Calotropis gigantea tender tissue as explant and carries out Fiber differentiation, obtains sprout primary；2) sprout primary for obtaining step 1) is cut into the stem section comprising 1~2 axillary bud, is placed in subculture medium and carries out squamous subculture, obtains tufted seedling；3) tufted seedling for obtaining step 2), which is placed on root media, carries out culture of rootage, obtains Calotropis gigantea tissue-culturing rapid propagation seedling.Make 4.83~5.93/25d of Calotropis gigantea growth coefficient using method of the invention；The glass rate of tissue-cultured seedling is reduced to 4.44~8.89%, and continuous multi-generation culture, glass rate maintains always 8% hereinafter, difference is not significant between each generation. |
| priorityDate | 2018-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 35.