http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108845148-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-535 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6854 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-577 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-535 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78 |
filingDate | 2018-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-02-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-108845148-B |
titleOfInvention | Cynoglossus semilaevis-derived exosome sandwich ELISA (enzyme-linked immuno sorbent assay) detection method and kit |
abstract | The invention discloses a cynoglossus semilaevis source exosome sandwich ELISA detection method and a kit, belongs to the technical field of fish biology, and particularly relates to the field of application of marine fish sperm exosome markers. The invention utilizes an anti-CD 63 antibody as a capture antibody and an anti-HSP 90 antibody as a detection antibody (enzyme-labeled antibody), develops a double-antibody sandwich ELISA method for detecting exosomes from cynoglossus semilaevis semen, provides a kit for identification and quantification, is used for identifying marker proteins of exosomes in cynoglossus semilaevis semen, and has important reference significance for sample exosomes from other body fluid sources of fish and identification of other marker proteins. The method has the advantages of strong specificity, high efficiency, simple and convenient operation, no need of large-scale instruments and expensive chemical reagents, and popularization value. |
priorityDate | 2018-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 22.