http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108845016-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-403 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-403 |
| filingDate | 2018-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108845016-B |
| titleOfInvention | Preparation method of L-cysteine self-powered biosensor |
| abstract | The invention discloses a preparation method of an L-cysteine (L-Cys) self-powered biosensor, which successfully combines an enzyme biofuel cell (EBFC) and a carbon cloth electrode and realizes the detection of L-cysteine by utilizing the change of an electric signal output by the EBFC. The design of self-powered biosensing is mainly focused on the anode of EBFC, and the aptamer rich in cytosine (C) is modified on the surface of the anode when Ag + When present, the C-C base pair in the aptamer can selectively capture Ag + A stable DNA double strand is formed, and a conjugate of modified glucose oxidase is fixed on the surface of an anode, so that the open-circuit voltage is increased. When the target L-Cys exists, the L-Cys is mixed with Ag + Insoluble thiolates are formed, part of the conjugate is detached and the signal is reduced. The open-circuit voltage value is in negative correlation with the concentration of the L-Cys, so that the detection of the L-Cys is realized. The sensor has the advantages of no need of an external power supply in the detection process, low cost, simple instrument and easy realization of miniaturization. |
| priorityDate | 2018-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 44.