http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108841929-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6883 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6883 |
| filingDate | 2018-06-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108841929-B |
| titleOfInvention | PCR amplification system and detection kit for human ATP7b gene exon and application method thereof |
| abstract | The invention belongs to the technical field of human nucleic acid in-vitro detection, and particularly relates to a PCR amplification detection system of a human ATP7b gene and application thereof. The PCR amplification detection system comprises: analyzing sequences of the primer group, the PCR reaction mother liquor container and the Sanger after Sequencing; the primer set container comprises primers SEQ ID No. 1-SEQ ID No.42 stock solutions. After the DNA extraction condition (the DNA quality meets the requirements that OD260/OD280 is more than or equal to 1.6 and less than or equal to 2.0, and the lowest concentration of the DNA is more than or equal to 10.0 ng/mu L), the fragments of 21 exon of human ATP7b gene are amplified simultaneously through PCR reaction, the judgment of whether the exon of ATP7b gene has deletion mutation is carried out through agarose gel electrophoresis of a target fragment, then the recovery and purification of electrophoresis gel DNA are carried out for one-generation sequencing, the gene Sequence of the sequencing fragment is compared with Reference Sequence (Reference Sequence in an authority database), and finally the judgment of whether the target Sequence has single base mutation is carried out, so that the attack cause of hepatolenticular nuclear degeneration on the molecular genetic level is researched and analyzed by a gold standard method on all the exons of the human ATP7b gene. Moreover, the method is simple to operate, has high popularization of instruments and equipment, and is suitable for popularization and use. |
| priorityDate | 2018-06-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 25.