http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108841924-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d7d5ab1291708c4e0c1ecef387d5dad5 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6834 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6834 |
| filingDate | 2018-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_56686ee1c7f07ffe90d965d50e2daf04 |
| publicationDate | 2018-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108841924-A |
| titleOfInvention | Rapid detection method of SNP sites in peripheral blood leukocytes |
| abstract | A method for rapid detection of SNP sites in peripheral blood leukocytes, characterized in that ammonium chloride solution, nanoporous balls, and hexaoligonucleotides are used as supplies for detection, and the detection of SNP sites in peripheral blood leukocytes is completed through the following steps Detection, the first step is to take the patient's EDTA anticoagulant blood sample; the second step is to separate and enrich the white blood cells; the third step is to cut and segment the enriched white blood cells; the fourth step is to decompose the double-stranded DNA of the white blood cells after segmentation Strand, to generate single-stranded DNA, using the base complementarity principle of DNA, to combine single-stranded DNA with fluorescently labeled hexaoligonucleotides to generate hybridized fragments: the fifth step, the hybridized fragments enter the nanopore ball, and use a fluorescence detector Perform detection, establish a complete set of gene fluorescent labeling maps, use a computer to calculate, and finally obtain the required SNP site genome sequence. The invention does not require a professional laboratory, does not require a PCR employment certificate, has low detection cost, simple process, short time and accurate result. |
| priorityDate | 2018-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 23.