http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108823196-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y501-02002 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-90 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-10 |
filingDate | 2018-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2022-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-108823196-B |
titleOfInvention | Preparation method of high-load immobilized mandelic acid racemase |
abstract | The invention discloses a preparation method of high-load immobilized mandelic acid racemase. The method comprises the following specific steps: 1) preparing a glucan grafted agarose medium; 2) activation of the medium; 3) bromoalcoholization of the medium; 4) mediator coupling of iminodiacetic acid; 5) the medium chelates divalent metal ions; 6) immobilized mandelic acid racemase. The method utilizes the characteristic that glucan molecules have much polyhydroxy to prepare the glucan grafted agarose medium chelating metal ions to immobilize the mandelic acid racemase and improve the carrying capacity of the racemase. The immobilized racemase prepared by the invention has better tolerance to temperature and pH and operation stability than the immobilized racemase obtained by taking glucan ungrafted agarose microspheres chelating metal ions as a medium. |
priorityDate | 2018-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 57.