http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108823179-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1096 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-001 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2018-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108823179-B |
| titleOfInvention | Transaminase derived from actinomycetes, mutant, recombinant bacterium and application |
| abstract | The invention discloses a transaminase, mutant and recombinant bacteria derived from actinomycetes and a biological preparation method of R-3-aminobutanol provided by the invention. The needed recombinant transaminase can be prepared in large quantities by constructing escherichia coli genetic engineering bacteria and then fermenting, and is relatively easy to obtain and cheap. The reaction is in a one-pot type, the substrate and the enzyme are added and then start to react, and the final product R-3-aminobutanol is directly obtained, so that the industrial cost is low. Compared with the wild type, the actinomycete transaminase mutant provided by the invention has better catalytic activity. Under an optimal system, the conversion rate of 100mM substrate reaches 90%, and the ee value reaches 99.9%; the conversion rate of 500mM substrate reaches 78%, the ee value reaches 99.9%, and compared with the wild type, the conversion rate of the substrate is respectively improved by 12% -25%. |
| priorityDate | 2018-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 273.