http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108815152-B
Outgoing Links
Predicate | Object |
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classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K31-255 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K9-0019 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K31-255 |
filingDate | 2018-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2021-04-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2021-04-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-108815152-B |
titleOfInvention | Construction method of azoospermia mouse model |
abstract | The invention discloses a method for constructing an azoospermia mouse model, which comprises the following steps: (1) providing a sexually mature male mouse with the weight of 25-35 g; (2) dissolving Busulfan in DMSO, and diluting with normal saline to obtain diluent; (3) disinfecting the lower abdomen of the mouse with alcohol, airing, sucking the diluent obtained in the step (2), inserting a needle, and finishing the first intraperitoneal injection after no error is confirmed by back pumping; and then injecting for 3 times at intervals in the same day, and obtaining the azoospermia mouse model after 35-37 days. The method has high molding rate and good stability; according to the invention, 84 accumulated C57BL/6 mice are modeled for azoospermia, the total number of the models is 84/84 and the molding rate is 100% through testis HE staining identification; by adopting the method for constructing the model, the death rate of the mouse is 0 percent; the total number of deaths of 84C 57BL/6 mice injected cumulatively according to the method of the present invention was 0, and the mortality was 0%. |
priorityDate | 2018-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 100.