http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108801996-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6486 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2018-06-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108801996-B |
| titleOfInvention | Method for detecting proportion of phosphorylated alpha synuclein positive cells |
| abstract | The invention discloses a method for detecting the proportion of phosphorylated alpha synuclein positive cells in peripheral blood neurons, which comprises the following steps: (1) taking a peripheral blood sample; (2) lysing erythrocytes in the blood sample; (3) separating out mixed cell population containing rare cells and a small amount of white blood cells; (4) sorting a population of cells enriched for neurons; (5) paving a board; (6) specific labeling; (7) carrying out fluorescence excitation on the specifically marked cells by using a laser confocal microscope, and photographing and storing data; (8) and analyzing the photographing result by using image J image analysis and statistics software to obtain the proportion of the phosphorylated alpha synuclein positive cells in peripheral blood neurons. The method detects the proportion of the phosphorylated alpha synuclein in peripheral blood neurons by an immunofluorescence chemical method, and is rapid, accurate and high in sensitivity. |
| priorityDate | 2018-06-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 59.