http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108801994-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2018-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-04-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-04-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108801994-B |
| titleOfInvention | Fluorescence detection method for tissue focal death and detection kit based on method |
| abstract | The invention provides a fluorescence detection method of tissue apoptosis and a detection kit based on the method. The fluorescence detection method of the present invention comprises: preparing frozen tissue sections, dropwise adding endogenous avidin to seal liquid A and liquid B, and sealing each sample according to the ratio of 2:3, dropwise adding TdT enzyme reaction liquid and Streptavidin-FITC labeled working solution, blocking with 5% BSA, and mixing each sample according to the weight ratio of 2:3, incubating Caspase-1 primary antibody and secondary antibody, staining a nucleus, sealing a piece, and carrying out fluorescence detection. All the processes are carried out in the dark. Under a fluorescence microscope, the phenomenon of the coexpression of TUNEL (green light) and Caspase-1 (red light) can be seen, and the coexpression cells are focal death cells and show the plaque-shaped fluorescence. The invention also discloses a kit suitable for the detection method, which is convenient to operate, quick and sensitive and has important significance in detecting the focusing and death diseases. |
| priorityDate | 2018-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 51.