http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108779442-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y605-01001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-93 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y605-01002 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate | 2017-02-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108779442-B |
| titleOfInvention | Compositions, systems and methods for various ligases |
| abstract | Provided herein are compositions, systems, and methods using a variety of ligases, wherein at least one of the ligases is an adenylation-deficient ATP-dependent ligase or a non-adenylated ATP-dependent ligase (eg, present in no ATP mix). In certain embodiments, multiple ligases are used to ligate a pre-adenylated double-stranded sequence to a non-adenylated double-stranded sequence (eg, the adenylation-deficient ATP-dependent ligase or non-adenylated double-stranded ligase). The acidified ATP-dependent ligase joins the first strand, and the second ligase joins the second strand). In other embodiments, provided herein are mutant T4 ligases (eg, a K159S mutant or a K159C mutant). |
| priorityDate | 2016-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 373.