http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108776185-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_489aa2cd1ae60fe92ef599b7516b721d |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-062 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-067 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 |
| filingDate | 2018-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dd440c26aff16911c98394c7dabe2518 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dc1d7bc122113dad9f70ef313e51be8a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_83741fa79f6e154b5a8ca065aa1320c0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d8431f28b382aa8841105ce895dfcd5b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c0eca2b7b9a7c46a28d481554526dd16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fe14e9c0f33bc841bc17205677715f2b |
| publicationDate | 2018-11-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108776185-A |
| titleOfInvention | A method for detection and analysis of hydroxyl-containing cholesterol and its metabolites |
| abstract | The invention relates to the field of analytical chemistry, in particular to a detection and analysis method for hydroxyl-containing cholesterol and its metabolites. The detection and analysis method is specifically: the stable isotope labeling derivatization reaction occurs between the hydroxyl-containing cholesterol and its metabolites and the derivatization reagent d 0 -/ d 3 -3 -N -methyl-2'-carboxyrhodamine 6G, and the magnetic dispersion solid The derivatized products obtained after phase extraction were filtered by a filter membrane and detected by an ultra-high performance liquid chromatography triple quadrupole tandem mass spectrometry system. The present invention uses a stable isotope labeling derivatization reagent with permanent positive charge d 0 ‑/ d 3 ‑MCR6G to label hydroxyl-containing cholesterol and metabolites in biological samples. The derivatization reaction conditions are mild and fast, and the derivatization efficiency is high. Magnetic dispersive solid-phase extraction technology improves extraction efficiency, reduces sample pretreatment time, can significantly improve chromatographic resolution of analytes and mass spectrometry ionization efficiency, and reduce matrix interference. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113237982-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109633141-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111057055-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111057055-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114487209-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114577949-A |
| priorityDate | 2018-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 100.