http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108774628-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-0258 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-245 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-108 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2018-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108774628-B |
| titleOfInvention | Escherichia coli engineering bacterium for synthesizing neonatal meningitis escherichia coli glycoprotein conjugate vaccine and application |
| abstract | The invention discloses an escherichia coli engineering bacterium for synthesizing an escherichia coli glycoprotein conjugate vaccine for neonatal meningitis and application thereof. Relates to a method for constructing a cell factory for synthesizing Escherichia coli O1 serotype glycoprotein conjugate vaccine for causing neonatal meningitis. The O1 antigen synthetic gene cluster plasmid is constructed by utilizing the efficient homologous recombination efficiency of saccharomyces cerevisiae and based on a DNA Assembler method. Transforming O1 antigen into shuttle plasmid of synthetic gene cluster in Escherichia coli JM109, extracting lipopolysaccharide, performing gel electrophoresis and identifying plasmid by silver staining; deletion in JM109 with the aid of FLP-FRT waaL And wecA and eliminating the interference of original incomplete O antigen. The pET28a (+) plasmid was engineered, induced, glycoprotein-binding vaccines were synthesized, glycoproteins were purified by means of the AKTA Primeplus protein purification workstation and identified by western-blotting. The recombinant escherichia coli constructed by the invention provides a new idea for synthesizing glycoprotein conjugate vaccine by a biological method. |
| priorityDate | 2018-07-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 389.