http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108753809-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-6418 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1029 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74 |
| filingDate | 2018-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108753809-B |
| titleOfInvention | Recombinant blue algae for efficiently expressing fatty acid and preparation method thereof |
| abstract | The invention discloses a recombinant blue algae for efficiently expressing fatty acid and a preparation method thereof, and relates to the field of fatty acid production. The recombinant blue algae is obtained by constructing a plasmid for improving the fatty acid expression quantity of the blue algae and transforming, and the recombinant blue algae over-expresses prolipoprotein diacyltransferase Lgt and thioesterase AcTesA. The prolipoprotein diacyltransferase Lgt is a scaffold protein and is positioned on a cell membrane under the action of a blue algae membrane guide sequence. The thioesterase AcTesA is connected with the scaffold protein Lgt through the flexible peptide FL3, has low substrate specificity, can hydrolyze acyl ACP of C8-C16, and can obtain abundant fatty acid products. According to the invention, the thioesterase protein AcTesA is fixed on the cell membrane through the scaffold protein Lgt, so that the synthesis speed of fatty acid in the blue algae cell is accelerated, the ROS content in the cell is reduced, and finally, the yield of fatty acid secreted outside the cell by the transgenic mutant strain is improved. |
| priorityDate | 2018-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 317.