abstract |
The present teachings provide modified enzymes, preferably phytases, with increased stability, which is assumed to be caused by increased glycosylation. The enzymes can be modified to introduce or increase the number of glycosylation sites in the amino acid sequence, or glycosylation can be increased by using specific host production methods, or both. Enzymes of the present teachings have increased stability after treatment at elevated temperatures, which can be measured by inactivation reversibility or percent recovery after treatment such as heating Measurement. Enzymes of the present teachings can be applied, for example, in food, feed and feed pelleting. |