http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108739380-B

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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-001
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-008
classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00
filingDate 2018-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2020-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2020-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-108739380-B
titleOfInvention Method for one-time seedling formation of rhizoma bletillae tissue culture seedlings
abstract The invention discloses a one-time seedling formation method of bletilla striata tissue culture seedlings, wherein the proliferation culture and rooting culture stages of the bletilla striata tissue culture seedlings are completed on a culture medium for sterile seeding, only a first culture medium is required to be prepared, and a proliferation inducer and a rooting and bulb-forming inducer are sequentially added into culture bottle seedlings, so that the bletilla striata tissue culture seedlings from seeds to the rooting and bulb-forming seedlings can be completed at one time, and the seedlings can be taken out of the bottle and hardened. Under the condition of not reducing the multiplication times, the tissue culture seedlings grow strongly and have developed root systems. Compared with the conventional tissue culture and rapid propagation technology, the culture medium is only required to be prepared once, and the culture medium is not required to be prepared and transferred in the propagation and rooting links, so that the cost of the culture medium is saved by more than 2 times, the time for a large number of bottle transfer inoculations is saved, the tissue culture procedure is simplified, the production cost of bletilla striata tissue culture seedlings can be greatly reduced, and the cost is saved by more than half. The method is simple to operate, low in production cost, capable of producing a large number of high-quality seedlings in a short time, and providing technical support and guarantee for breeding the high-quality and low-price seedlings of bletilla striata.
priorityDate 2018-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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