| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_838354375423711d1f8239a99eed7c87 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-56 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-66
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-66
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65 |
| filingDate |
2018-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9a43fe1fa505ddced80efec5432219bc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_98323423e38505fa7dcfb1caefb18e30
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_950a339b3031dcbfa58a81837713e22b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4730c9b574d7ba8855595809ff35d216
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_73320336d8720b77476e08fb1511f79f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a3f5178dfe2f99aab173937edfc17645 |
| publicationDate |
2018-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-108728514-A |
| titleOfInvention |
Chemical-activated luciferase gene expression chicken interferon α biological activity detection methods |
| abstract |
The invention discloses a kind of chemical-activated luciferase gene expression chicken interferon α biological activities detection method and its applications.This approach includes the following steps：The genetic fragment of the pMx1 of chicken Mx1 albumen is obtained using PCR amplification；The genetic fragment of pMx1 is inserted into the ends 5' of pGL3-basic carrier luc genes, then pMx1-luc fusion segments are obtained using PCR amplification；The genetic fragment of the pCMV and EGFP in pEGFP-N1 carriers are substituted with pMx1-luc fusion segments, build pMx1-luc plasmids, transfectional cell, and select stable transfected cells strain；Colonized culture is carried out to the stable transfected cells strain filtered out；Standard curve is prepared with the chicken interferon α standard items of gradient dilution, is incubated altogether in cell chicken interferon α samples to be checked being added after colonized culture, then fluorescence intensity, the potency of combined standard curve evaluation chicken interferon α samples to be checked. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110951761-A |
| priorityDate |
2017-12-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |