| abstract |
The invention relates to a method for controllably immobilizing bioactive factors by light-generated aldehyde/ketone groups on the material surface/interface. The method of the present invention does not require additional chemical modification of the bioactive factor to be immobilized, does not destroy the biological activity of the bioactive factor, and can perform mild and effective covalent immobilization on the material surface/interface under the control of light at low temperature or room temperature. The method for immobilizing biologically active factors of the present invention has simple process and good repeatability, and can realize the immobilization of proteins, polypeptides, antibodies, growth factors or enzymes on the surfaces of various glasses, metals, organic polymers, etc., and is an innovation of protein immobilization methods . The present invention further relates to the application of the obtained protein immobilization material in various fields of biomedicine and tissue engineering, such as cell scaffold, in vitro diagnosis, enzyme catalysis, and protein purification. |