http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108722369-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J20-268 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-08 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J20-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J20-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-08 |
| filingDate | 2018-05-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108722369-B |
| titleOfInvention | Universal convenient epitope imprinting method and application of obtained molecularly imprinted polymer |
| abstract | The invention discloses a universal convenient epitope imprinting method, which comprises the following steps: firstly, selecting terminal polypeptide of a target protein as a characteristic epitope, obtaining glycosylated polypeptide after saccharification treatment as an imprinting template, anchoring the imprinting template to a boric acid functionalized substrate material through boron affinity, then selecting monomer silanization reagents with different types and proportions to carry out non-covalent imprinting according to the amino acid type of the epitope sequence, and realizing accurate imprinting by adjusting imprinting time on the imprinting layer thickness to obtain the molecularly imprinted polymer. The imprinting technology has the advantages of good universality, convenient preparation, good identification performance and the like. The molecular imprinting material prepared by the invention can not only identify epitope fragments, but also can specifically identify complete target protein, and has important application prospect in the fields of separation, enrichment, purification, sensing, proteomics, target identification and the like. |
| priorityDate | 2017-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 257.