http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108719056-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-001 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01H4-008 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01G31-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01G31-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
| filingDate | 2018-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108719056-B |
| titleOfInvention | Tissue culture seedling method of sea buckthorn |
| abstract | The invention provides a tissue culture seedling method of sea buckthorn, which comprises the following steps: (1) selecting materials: taking tender stem tips as tissue culture seedling raising materials; (2) sterilizing the tender stem tips; (3) determining culture conditions; (4) induction of tender stem tips; (5) proliferation of tender stem tips; (6) rooting of the tender stem tip; (7) transplanting: inducing rooting in rooting culture medium for 40-60 days, transplanting, culturing in matrix, controlling humidity to over 95% in the first week, gradually prolonging ventilation and illumination time, and naturally ventilating completely for 14-16 days; meanwhile, the temperature is controlled well, the temperature is controlled to be 25 +/-1 ℃ in the daytime and 15-18 ℃ at night. The tissue culture seedling method of the sea-buckthorn can effectively improve the induction rate, the proliferation rate and the rooting rate of the tender stem tips of the sea-buckthorn in the tissue culture process, and the survival rate after transplanting is over 96 percent. |
| priorityDate | 2018-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 20.