http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108709997-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-91215 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-573 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-577 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-573 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-577 |
| filingDate | 2018-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108709997-B |
| titleOfInvention | Substrate search method for LRR receptor kinase |
| abstract | The invention provides a substrate searching method of LRR receptor kinase, which comprises the following steps: s1: incubating an LRR receptor kinase with ATP- γ -S to obtain a first mixture; s2: incubating the first mixture with total protein of the tissue enriched for the LRR receptor kinase to obtain a second mixture; s3: incubating the second mixture with PNBM to obtain a third mixture; s4: removing PNBM in the third mixture to obtain a fourth mixture; s5: incubating the fourth mixture with pretreated protein G agarose and centrifuging to obtain a first supernatant; s6: incubating the phosphorothioate-resistant monoclonal antibody coupled with protein G agarose with the first supernatant to obtain a first precipitate; s7: analyzing the first precipitate to determine a substrate for the LRR receptor kinase. |
| priorityDate | 2018-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 60.