http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108707644-B

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classificationCPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6832
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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6832
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806
filingDate 2018-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2021-06-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2021-06-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-108707644-B
titleOfInvention Method for detecting pyrophosphate and alkaline phosphohydrolase based on DNA templated copper cluster probe
abstract The invention relates to a method for detecting pyrophosphate ions and alkaline phosphohydrolase by using a DNA (deoxyribonucleic acid) templated copper cluster probe, belonging to the technical field of nano biosensing. The method comprises the following steps: synthesizing a copper cluster probe emitting red fluorescence by using DNA as a template, and then passing through Cr 3+ The fluorescent copper cluster nanometer switch triggered by the ions realizes the high-sensitivity and high-selectivity detection of the focusing phosphate ions and the alkaline phosphohydrolase. In addition, the fluorescent nano probe can also realize the detection of pyrophosphate ions and alkaline phosphohydrolase in a complex system. The DNA templated copper cluster probe has the advantages of mild synthesis conditions, simple synthesis steps, stable fluorescence and high biocompatibility, and the probe has the advantages of rapidness, good selectivity, high sensitivity and the like for detecting pyrophosphate ions and alkaline phosphohydrolase.
priorityDate 2018-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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