| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d55dd4da0d8f5bfa72abe0507617311d |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-154 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0071
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6872
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y114-11 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6827
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 |
| filingDate |
2016-10-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dbd401488e45b804b705ec988026ff4e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b0e4f19dcca6f7386add818ef77f4853
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_df1f6a598da24d413682e770da4a55f8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d7d26db9be40f4d1f20599756e105d39
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c21760d4b25a3b003ee28c2a9dd74a68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7bc55e6d0f3bca3721351df0f66d6aa1 |
| publicationDate |
2018-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-108699598-A |
| titleOfInvention |
Compositions and methods for determining modified cytosines by sequencing |
| abstract |
Methods are provided for identifying the location and phasing of modified cytosines (C) in long nucleic acids. In some embodiments, the method may comprise (a) reacting a first portion of a nucleic acid sample containing at least one C and/or at least one modified C with DNA glucosyltransferase and cytidine deaminase to produce a second a product and optionally reacting a second portion of the sample with dioxygenase and cytidine deaminase to produce a second product; and (b) combining the first and optionally second obtained from (a) The sequences of the products or amplification products thereof are compared to each other and/or to an unprocessed reference sequence to determine which Cs in the original nucleic acid fragment are modified. Also provided are modified TET methylcytosine dioxygenases that more efficiently convert methylcytosine to carboxymethylcytosine. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113088562-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113846070-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113846070-B
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115323035-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110982883-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110358817-A |
| priorityDate |
2015-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |