| abstract |
The present invention relates to a process for obtaining immobilized enzyme preparations, in particular to the preparation and use of novel active conjugates of enzymes with polymeric carriers. The conjugate has the properties of Longidaza medicaments, also has the properties of anti-inflammatory action and inhibition of connective tissue hyperplasia, can be used for manufacturing stable, active and safe long-acting medicaments in the form of suppositories, ointments, injections or cosmetic creams, and can also be used for manufacturing veterinary medicaments. The method comprises conjugating hyaluronidase to a water-soluble copolymer using the carbodiimide method or the azide method. The conjugation is carried out using a copolymer of N-oxide 1, 4-vinylpiperazine, (N-carboxymethyl) -1, 4-vinylpiperazine or its hydrazide and 1, 4-vinylpiperazine, of the general formula Wherein n is from 40% to 90% of the total number of units; m accounts for 3% to 40% of the total number of units; n + m + l is 100% of the total number of units. |